康新华,韩庆彦,毋艳萍,牛琼,赵雯,高军军

• 1:甘肃省动物疫病预防控制中心

摘要(Abstract)：

为建立适用于小反刍兽疫病毒（PPRV）的临床快速检测方法，本研究基于PPRV N基因序列设计并筛选引物与探针，通过优化逆转录-重组酶聚合酶扩增（RT-RPA）反应体系的温度和时间，联合侧向层析试纸条（LFD）构建RT-RPA-LFD检测方法，并系统评价其性能。结果表明，该方法在42℃反应15 min即可完成扩增，并可通过LFD实现结果的可视化。其灵敏度为10拷贝/μL，与常规RT-PCR灵敏度相当。对山羊传染性胸膜肺炎、绵羊支原体和丝状支原体山羊亚种等其他病原体无交叉，批间和批内重复试验均呈现出理想的检测结果，且临床样本检测结果与荧光RT-PCR检测结果的符合率达100%。综上所述，本研究建立的RT-RPA-LFD检测方法用时短、操作便捷、兼具高灵敏度与强特异性，且重复性好，适用于小反刍兽疫病毒的现场快速检测。

关键词(KeyWords)： 小反刍兽疫病毒;重组聚合酶扩增;侧向层析试纸条;快速检测

Abstract:

Keywords:

基金项目(Foundation): 甘肃省农业农村厅科技项目（GNKJ-2024-35）

作者(Author): 康新华,韩庆彦,毋艳萍,牛琼,赵雯,高军军

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